Analysis of all plates
Sam Hunter
18 March, 2021
NOTE: only the plates in the following list are included in this report.
## TRIMMER0002_P2
## TRIMMER0003_P3
## TRIMMER0004.2_P4
## TRIMMER0005_P5
## TRIMMER0006_P6
## TRIMMER0007_P7
## TRIMMER0010_P10R
## TRIMMER0013_P28
## TRIMMER0014_P29
## TRIMMER0015_P30
## TRIMMER0016_P31
## TRIMMER0017_P45
## TRIMMER0018_P8
## TRIMMER0019_P9
## TRIMMER0020_P10
## TRIMMER0021_P11
## TRIMMER0022_P12
## TRIMMER0023_P13
## TRIMMER0024_P14
## TRIMMER0026_P16
## TRIMMER0027_P33
## TRIMMER0028_P34
## TRIMMER0029_P35
## TRIMMER0030_P36
## TRIMMER0031_P15
## TRIMMER0033_P17
## TRIMMER0034_P18
## TRIMMER0035_P19
## TRIMMER0036_P20
## TRIMMER0037_P21
## TRIMMER0038_P22
## TRIMMER0039_P23
## TRIMMER0040_P24
## TRIMMER0041_P25
## TRIMMER0042_P26
## TRIMMER0043_P27
## TRIMMER0044_P37
## TRIMMER0045_P38
## TRIMMER0046_P39
## TRIMMER0047_P40
## TRIMMER0048_P41
## TRIMMER0049_P42
## TRIMMER0050_P43
## TRIMMER0051_P44
## TRIMMER0052_P46
## TRIMMER0053_P47
## TRIMMER0054_P48
## TRIMMER0055_P49
## TRIMMER0056_P50
## TRIMMER0057_P51
## TRIMMER0058_P52
## TRIMMER0059_P54
## TRIMMER0060_P55
## TRIMMER0061_P56
## TRIMMER0062_P57
## TRIMMER0063_P58
## TRIMMER0064_P59
## TRIMMER0065_P60
## TRIMMER0066_P65
## TRIMMER0067_P66
## TRIMMER0068_P1R
## TRIMMER0069_PDP1
## TRIMMER0070_PDP2
## TRIMMER0073_PS1
## TRIMMER0074_PS2
## TRIMMER0075_PS3
## TRIMMER0077_P32R
## TRIMMER0078_P67
## TRIMMER0079_P68
## TRIMMER0080_P69
## TRIMMER0076_P6R
## TRIMMER0081_P70
## TRIMMER0082_P71
## TRIMMER0083_P72
## TRIMMER0084_P73
## TRIMMER0085_P74
## TRIMMER0086_P75## ERROR: Annotated results have Sample_Names that are missing from metadata file.Summary statistics by funding period
Funding periods Year1 = July 1 2019 - June 30 2020 Year2 = July 1 2020 - June 30, 2021 Year3 = July 1 2021 - June 30, 2022
Within each of the above date ranges, report a count of: 1) total number of monoclonal hybridomas sequenced 2) how many of these yielded only one heavy and one light chain sequence (as annotated by ANARCI) 3) how many of these had more than one heavy or light chain (or both) 4) total number of oligoclonal/parent hybridomas sequenced
Total plates sequenced per funding year:
| Var1 | Freq |
|---|---|
| Year1 | 59 |
| Year2 | 18 |
Total number of monoclonal, oligoclonal, and control samples sequenced per funding year.
| Year1 | Year2 | |
|---|---|---|
| monoclonal | 3838 | 1371 |
| oligoclonal | 1591 | 295 |
| PositiveControl | 106 | 16 |
| NegativeControl | 129 | 46 |
Summarized sequences (ASVs) identified per sample for all funding years.
Values are number of samples that fall within each category.
Note that these categories were generated using the number of sequences identified in each sample where ANARCI could categorize the resulting sequence and predict domains.
| monoclonal | oligoclonal | PositiveControl | NegativeControl | |
|---|---|---|---|---|
| LC=0 and HC=0 | 635 | 129 | 59 | 168 |
| LC=0 and HC=1 | 35 | 6 | 0 | 0 |
| LC=1 and HC=0 | 352 | 29 | 3 | 4 |
| LC=0 and HC>1 | 1 | 30 | 0 | 0 |
| LC>1 and HC=0 | 85 | 38 | 0 | 0 |
| LC=1 and HC=1 | 2916 | 270 | 31 | 1 |
| LC>1 or HC>1 (both > 0) | 1185 | 1384 | 29 | 2 |
Summarized sequences by Sample Category
Sample Categories include:
- NeuroMab mAbs
- Non-NeuroMab mAbs
- NeuroMab Alternative Subclones
- Lead oligoclonal Abs
Monoclonal samples:
| 0 | 1 | 2 | 3 | 4 | |
|---|---|---|---|---|---|
| LC=0 and HC=0 | 0 | 40 | 495 | 100 | 0 |
| LC=0 and HC=1 | 0 | 4 | 28 | 3 | 0 |
| LC=0 and HC>1 | 0 | 0 | 1 | 0 | 0 |
| LC=1 and HC=0 | 1 | 12 | 297 | 42 | 0 |
| LC=1 and HC=1 | 5 | 404 | 1818 | 688 | 1 |
| LC>1 and HC=0 | 0 | 1 | 65 | 19 | 0 |
| LC>1 or HC>1 (both > 0) | 36 | 112 | 714 | 323 | 0 |
Oligoclonal samples:
| 0 | 4 | |
|---|---|---|
| LC=0 and HC=0 | 31 | 98 |
| LC=0 and HC=1 | 2 | 4 |
| LC=0 and HC>1 | 0 | 30 |
| LC=1 and HC=0 | 2 | 27 |
| LC=1 and HC=1 | 173 | 97 |
| LC>1 and HC=0 | 2 | 36 |
| LC>1 or HC>1 (both > 0) | 79 | 1305 |
Usable as is oligoclonal samples: These are samples that were originally flagged as likely to be oligoclonal, but produced only a single HC and LC, suggesting that they are probably monoclonal.
| sample_name | trimmer_id | Category | LCs.Annotated | HCs.Annotated | LC.K.Called | HC.H.Called | |
|---|---|---|---|---|---|---|---|
| 350 | P45_C1 | L118/28 | 4 | 1 | 1 | 1 | 1 |
| 1411 | P45_F2 | L118/73 | 4 | 1 | 1 | 1 | 1 |
| 2811 | P45_D4 | L118/48 | 4 | 1 | 1 | 1 | 1 |
| 3411 | P45_B5 | L118/20 | 4 | 1 | 1 | 1 | 1 |
| 5811 | P45_B8 | L118/23 | 4 | 1 | 1 | 1 | 1 |
| 6115 | P45_E8 | L118/68 | 4 | 1 | 1 | 1 | 1 |
| 8511 | P45_E11 | L118/70 | 4 | 1 | 1 | 1 | 1 |
| 2942 | P43_E4 | L21/14 | 4 | 1 | 1 | 1 | 1 |
| 3842 | P43_F5 | N486/7 | 4 | 1 | 1 | 1 | 1 |
| 4442 | P43_D6 | N34/1 | 4 | 1 | 1 | 1 | 1 |
| 5244 | P43_D7 | N34/17 | 4 | 1 | 1 | 1 | 1 |
| 6842 | P43_D9 | N34/31 | 4 | 1 | 1 | 1 | 1 |
| 4164 | P44_D1 | K28/9 | 4 | 1 | 1 | 1 | 1 |
| 1543 | P44_G2 | N279A/16 | 4 | 1 | 1 | 1 | 1 |
| 1743 | P44_A3 | K28/92 | 4 | 1 | 1 | 1 | 1 |
| 1843 | P44_B3 | K28/33 | 4 | 1 | 1 | 1 | 1 |
| 3943 | P44_G5 | N279A/40 | 4 | 1 | 1 | 1 | 1 |
| 4245 | P44_B6 | K28/30 | 4 | 1 | 1 | 1 | 1 |
| 6543 | P44_A9 | K28/45 | 4 | 1 | 1 | 1 | 1 |
| 6643 | P44_B9 | K28/26 | 4 | 1 | 1 | 1 | 1 |
| 6843 | P44_D9 | K28/1 | 4 | 1 | 1 | 0 | 1 |
| 7165 | P44_G9 | N327/45 | 4 | 1 | 1 | 1 | 1 |
| 8343 | P44_C11 | K28/11 | 4 | 1 | 1 | 1 | 1 |
| 8643 | P44_F11 | L114/22 | 4 | 1 | 1 | 1 | 1 |
| 5845 | P47_B8 | L124/47 | 4 | 1 | 1 | 0 | 1 |
| 3347 | P49_A5 | K20/58 | 4 | 1 | 1 | 1 | 1 |
| 4173 | P49_A6 | K20/59 | 4 | 1 | 1 | 1 | 1 |
| 4947 | P49_A7 | K20/74 | 4 | 1 | 1 | 1 | 1 |
| 5747 | P49_A8 | K20/82 | 4 | 1 | 1 | 1 | 1 |
| 6547 | P49_A9 | K20/83 | 4 | 1 | 1 | 1 | 1 |
| 6847 | P49_D9 | L127/74 | 4 | 1 | 1 | 1 | 1 |
| 7249 | P49_H9 | K20/86 | 4 | 1 | 1 | 1 | 1 |
| 8947 | P49_A12 | K20/91 | 4 | 1 | 1 | 1 | 1 |
| 7174 | P50_G1 | N437/23 | 4 | 1 | 1 | 1 | 1 |
| 1175 | P50_C2 | N275/31 | 4 | 1 | 1 | 1 | 1 |
| 2648 | P50_B4 | N275/12 | 4 | 1 | 1 | 1 | 1 |
| 2948 | P50_E4 | N69/17 | 4 | 1 | 1 | 1 | 1 |
| 3548 | P50_C5 | N275/35 | 4 | 1 | 1 | 1 | 1 |
| 4048 | P50_H5 | L107/68 | 4 | 1 | 1 | 1 | 1 |
| 4448 | P50_D6 | N269/30 | 4 | 1 | 1 | 1 | 1 |
| 5948 | P50_C8 | N275/38 | 4 | 1 | 1 | 0 | 1 |
| 6348 | P50_G8 | N437/81 | 4 | 1 | 1 | 1 | 1 |
| 6448 | P50_H8 | K36/5 | 4 | 1 | 1 | 1 | 1 |
| 6648 | P50_B9 | N275/20 | 4 | 1 | 1 | 1 | 1 |
| 6748 | P50_C9 | N275/41 | 4 | 1 | 1 | 1 | 1 |
| 7250 | P50_H9 | L107/86 | 4 | 1 | 1 | 1 | 1 |
| 7348 | P50_A10 | N275/3 | 4 | 1 | 1 | 1 | 1 |
| 7648 | P50_D10 | N282A/23 | 4 | 1 | 1 | 1 | 1 |
| 8250 | P50_B11 | N275/25 | 4 | 1 | 1 | 1 | 1 |
| 9448 | P50_F12 | N437/9 | 4 | 1 | 1 | 1 | 1 |
| 7182 | P55_G1 | L117/85 | 4 | 1 | 1 | 1 | 1 |
| 6652 | P55_B9 | L117/19 | 4 | 1 | 1 | 1 | 1 |
| 6852 | P55_D9 | L117/45 | 4 | 1 | 1 | 1 | 1 |
| 8952 | P55_A12 | L113/120 | 4 | 1 | 1 | 1 | 1 |
| 6853 | P56_D9 | L119/34 | 4 | 1 | 1 | 1 | 1 |
| 7453 | P56_B10 | L117/131 | 4 | 1 | 1 | 1 | 1 |
| 8185 | P56_A11 | L117/9 | 4 | 1 | 1 | 1 | 1 |
| 1454 | P57_F2 | L120/46 | 4 | 1 | 1 | 1 | 1 |
| 2187 | P57_E3 | L120/35 | 4 | 1 | 1 | 1 | 1 |
| 2454 | P57_H3 | L120/71 | 4 | 1 | 1 | 1 | 1 |
| 2654 | P57_B4 | L119/144 | 4 | 1 | 1 | 1 | 1 |
| 2854 | P57_D4 | L120/22 | 4 | 1 | 1 | 1 | 1 |
| 2954 | P57_E4 | L120/36 | 4 | 1 | 1 | 1 | 1 |
| 3654 | P57_D5 | L120/23 | 4 | 1 | 1 | 1 | 1 |
| 3854 | P57_F5 | L120/49 | 4 | 1 | 1 | 1 | 1 |
| 4054 | P57_H5 | L120/74 | 4 | 1 | 1 | 1 | 1 |
| 4354 | P57_C6 | L120/11 | 4 | 1 | 1 | 1 | 1 |
| 4854 | P57_H6 | L120/75 | 4 | 1 | 1 | 1 | 1 |
| 5354 | P57_E7 | L120/39 | 4 | 1 | 1 | 1 | 1 |
| 5454 | P57_F7 | L120/51 | 4 | 1 | 1 | 1 | 1 |
| 6354 | P57_G8 | L120/63 | 4 | 1 | 1 | 1 | 1 |
| 8454 | P57_D11 | L120/31 | 4 | 1 | 1 | 1 | 1 |
| 9255 | P57_D12 | L120/32 | 4 | 1 | 1 | 1 | 1 |
| 9354 | P57_E12 | L120/44 | 4 | 1 | 1 | 1 | 1 |
| 3188 | P58_C1 | L120/101 | 4 | 1 | 1 | 1 | 1 |
| 1055 | P58_B2 | L120/88 | 4 | 1 | 1 | 1 | 1 |
| 2755 | P58_C4 | L120/104 | 4 | 1 | 1 | 1 | 1 |
| 3055 | P58_F4 | L120/140 | 4 | 1 | 1 | 1 | 1 |
| 3455 | P58_B5 | L120/93 | 4 | 1 | 1 | 1 | 1 |
| 3655 | P58_D5 | L120/117 | 4 | 1 | 1 | 1 | 1 |
| 4257 | P58_B6 | L120/94 | 4 | 1 | 1 | 1 | 1 |
| 4655 | P58_F6 | L120/142 | 4 | 1 | 1 | 1 | 1 |
| 6055 | P58_D8 | L120/120 | 4 | 1 | 1 | 1 | 1 |
| 6555 | P58_A9 | L120/82 | 4 | 1 | 1 | 1 | 1 |
| 9160 | P58_C12 | L120/112 | 4 | 1 | 1 | 1 | 1 |
| 6190 | P59_F1 | L122/23 | 4 | 1 | 1 | 1 | 1 |
| 2258 | P59_F3 | L122/26 | 4 | 1 | 1 | 1 | 1 |
| 6056 | P59_D8 | L121/144 | 4 | 1 | 1 | 1 | 1 |
| 1193 | P60_C2 | L122/67 | 4 | 1 | 1 | 1 | 1 |
| 3259 | P60_H4 | L122/134 | 4 | 1 | 1 | 1 | 1 |
| 4857 | P60_H6 | L122/136 | 4 | 1 | 1 | 1 | 1 |
| 5193 | P60_C7 | L122/74 | 4 | 1 | 1 | 1 | 1 |
| 5957 | P60_C8 | L122/75 | 4 | 1 | 1 | 1 | 1 |
| 7457 | P60_B10 | L122/63 | 4 | 1 | 1 | 1 | 1 |
| 7195 | P65_G9 | N29/1 | 4 | 1 | 1 | 1 | 1 |
| 9458 | P65_F12 | N34/35 | 4 | 1 | 1 | 1 | 1 |
| 9174 | PDP2_C12 | L119/87 | 4 | 1 | 1 | 1 | 1 |
| 1279 | P73_A1 | NA | 0 | 1 | 1 | 1 | 1 |
| 2279 | P73_B1 | NA | 0 | 1 | 1 | 1 | 1 |
| 3279 | P73_C1 | NA | 0 | 1 | 1 | 1 | 1 |
| 7279 | P73_G1 | NA | 0 | 1 | 1 | 0 | 1 |
| 8279 | P73_H1 | NA | 0 | 1 | 1 | 1 | 1 |
| 9197 | P73_A2 | NA | 0 | 1 | 1 | 1 | 1 |
| 1074 | P73_B2 | NA | 0 | 1 | 1 | 1 | 1 |
| 11113 | P73_C2 | NA | 0 | 1 | 1 | 1 | 1 |
| 1474 | P73_F2 | NA | 0 | 1 | 1 | 1 | 1 |
| 1574 | P73_G2 | NA | 0 | 1 | 1 | 0 | 1 |
| 1674 | P73_H2 | NA | 0 | 1 | 1 | 1 | 1 |
| 1774 | P73_A3 | NA | 0 | 1 | 1 | 1 | 1 |
| 1874 | P73_B3 | NA | 0 | 1 | 1 | 1 | 1 |
| 1974 | P73_C3 | NA | 0 | 1 | 1 | 1 | 1 |
| 2280 | P73_F3 | NA | 0 | 1 | 1 | 1 | 1 |
| 2374 | P73_G3 | NA | 0 | 1 | 1 | 1 | 1 |
| 2574 | P73_A4 | NA | 0 | 1 | 1 | 1 | 1 |
| 2674 | P73_B4 | NA | 0 | 1 | 1 | 1 | 1 |
| 2874 | P73_D4 | NA | 0 | 1 | 1 | 1 | 1 |
| 3280 | P73_H4 | NA | 0 | 1 | 1 | 1 | 1 |
| 3374 | P73_A5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3474 | P73_B5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3574 | P73_C5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3774 | P73_E5 | NA | 0 | 1 | 1 | 1 | 1 |
| 4280 | P73_B6 | NA | 0 | 1 | 1 | 1 | 1 |
| 4374 | P73_C6 | NA | 0 | 1 | 1 | 1 | 1 |
| 4474 | P73_D6 | NA | 0 | 1 | 1 | 1 | 1 |
| 5074 | P73_B7 | NA | 0 | 1 | 1 | 1 | 1 |
| 51113 | P73_C7 | NA | 0 | 1 | 1 | 1 | 1 |
| 5280 | P73_D7 | NA | 0 | 1 | 1 | 1 | 1 |
| 5374 | P73_E7 | NA | 0 | 1 | 1 | 1 | 1 |
| 5474 | P73_F7 | NA | 0 | 1 | 1 | 1 | 1 |
| 5674 | P73_H7 | NA | 0 | 1 | 1 | 1 | 1 |
| 5974 | P73_C8 | NA | 0 | 1 | 1 | 1 | 1 |
| 6280 | P73_F8 | NA | 0 | 1 | 1 | 1 | 1 |
| 6374 | P73_G8 | NA | 0 | 1 | 1 | 1 | 1 |
| 6674 | P73_B9 | NA | 0 | 1 | 1 | 1 | 1 |
| 6774 | P73_C9 | NA | 0 | 1 | 1 | 1 | 1 |
| 71113 | P73_G9 | NA | 0 | 1 | 1 | 0 | 1 |
| 7474 | P73_B10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7674 | P73_D10 | NA | 0 | 1 | 1 | 1 | 1 |
| 8074 | P73_H10 | NA | 0 | 1 | 1 | 1 | 1 |
| 8374 | P73_C11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8674 | P73_F11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8874 | P73_H11 | NA | 0 | 1 | 1 | 1 | 1 |
| 9198 | P73_C12 | NA | 0 | 1 | 1 | 1 | 1 |
| 9474 | P73_F12 | NA | 0 | 1 | 1 | 1 | 1 |
| 2281 | P74_B1 | NA | 0 | 1 | 1 | 1 | 1 |
| 4281 | P74_D1 | NA | 0 | 1 | 1 | 1 | 1 |
| 5281 | P74_E1 | NA | 0 | 1 | 1 | 1 | 1 |
| 6281 | P74_F1 | NA | 0 | 1 | 1 | 1 | 1 |
| 8281 | P74_H1 | NA | 0 | 1 | 1 | 1 | 1 |
| 9199 | P74_A2 | NA | 0 | 1 | 1 | 1 | 1 |
| 1075 | P74_B2 | NA | 0 | 1 | 1 | 1 | 1 |
| 1475 | P74_F2 | NA | 0 | 1 | 1 | 1 | 1 |
| 1775 | P74_A3 | NA | 0 | 1 | 1 | 1 | 1 |
| 1875 | P74_B3 | NA | 0 | 1 | 1 | 1 | 1 |
| 1975 | P74_C3 | NA | 0 | 1 | 1 | 1 | 1 |
| 2075 | P74_D3 | NA | 0 | 1 | 1 | 1 | 1 |
| 2282 | P74_F3 | NA | 0 | 1 | 1 | 1 | 1 |
| 2375 | P74_G3 | NA | 0 | 1 | 1 | 1 | 1 |
| 2575 | P74_A4 | NA | 0 | 1 | 1 | 1 | 1 |
| 2675 | P74_B4 | NA | 0 | 1 | 1 | 1 | 1 |
| 2775 | P74_C4 | NA | 0 | 1 | 1 | 1 | 1 |
| 2875 | P74_D4 | NA | 0 | 1 | 1 | 1 | 1 |
| 3075 | P74_F4 | NA | 0 | 1 | 1 | 1 | 1 |
| 3375 | P74_A5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3475 | P74_B5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3675 | P74_D5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3775 | P74_E5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3975 | P74_G5 | NA | 0 | 1 | 1 | 1 | 1 |
| 41114 | P74_A6 | NA | 0 | 1 | 1 | 1 | 1 |
| 4282 | P74_B6 | NA | 0 | 1 | 1 | 1 | 1 |
| 4475 | P74_D6 | NA | 0 | 1 | 1 | 1 | 1 |
| 4675 | P74_F6 | NA | 0 | 1 | 1 | 1 | 1 |
| 5075 | P74_B7 | NA | 0 | 1 | 1 | 1 | 1 |
| 5282 | P74_D7 | NA | 0 | 1 | 1 | 1 | 1 |
| 5475 | P74_F7 | NA | 0 | 1 | 1 | 0 | 1 |
| 5775 | P74_A8 | NA | 0 | 1 | 1 | 1 | 1 |
| 5875 | P74_B8 | NA | 0 | 1 | 1 | 1 | 1 |
| 5975 | P74_C8 | NA | 0 | 1 | 1 | 1 | 1 |
| 6075 | P74_D8 | NA | 0 | 1 | 1 | 1 | 1 |
| 61114 | P74_E8 | NA | 0 | 1 | 1 | 1 | 1 |
| 6282 | P74_F8 | NA | 0 | 1 | 1 | 1 | 1 |
| 6675 | P74_B9 | NA | 0 | 1 | 1 | 1 | 1 |
| 6775 | P74_C9 | NA | 0 | 1 | 1 | 1 | 1 |
| 6875 | P74_D9 | NA | 0 | 1 | 1 | 1 | 1 |
| 6975 | P74_E9 | NA | 0 | 1 | 1 | 1 | 1 |
| 7075 | P74_F9 | NA | 0 | 1 | 1 | 1 | 1 |
| 7375 | P74_A10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7475 | P74_B10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7575 | P74_C10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7675 | P74_D10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7775 | P74_E10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7875 | P74_F10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7975 | P74_G10 | NA | 0 | 1 | 1 | 1 | 1 |
| 81114 | P74_A11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8282 | P74_B11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8375 | P74_C11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8575 | P74_E11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8675 | P74_F11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8975 | P74_A12 | NA | 0 | 1 | 1 | 1 | 1 |
| 9075 | P74_B12 | NA | 0 | 1 | 1 | 1 | 1 |
| 91100 | P74_C12 | NA | 0 | 1 | 1 | 1 | 1 |
| 9276 | P74_D12 | NA | 0 | 1 | 1 | 1 | 1 |
| 9375 | P74_E12 | NA | 0 | 1 | 1 | 1 | 1 |
| 9475 | P74_F12 | NA | 0 | 1 | 1 | 1 | 1 |
| 9575 | P74_G12 | NA | 0 | 1 | 1 | 1 | 1 |
| 2283 | P75_B1 | NA | 0 | 1 | 1 | 1 | 1 |
| 4283 | P75_D1 | NA | 0 | 1 | 1 | 1 | 1 |
| 5283 | P75_E1 | NA | 0 | 1 | 1 | 1 | 1 |
| 7283 | P75_G1 | NA | 0 | 1 | 1 | 1 | 1 |
| 8283 | P75_H1 | NA | 0 | 1 | 1 | 1 | 1 |
| 11115 | P75_C2 | NA | 0 | 1 | 1 | 1 | 1 |
| 1284 | P75_D2 | NA | 0 | 1 | 1 | 1 | 1 |
| 1576 | P75_G2 | NA | 0 | 1 | 1 | 1 | 1 |
| 1676 | P75_H2 | NA | 0 | 1 | 1 | 1 | 1 |
| 1776 | P75_A3 | NA | 0 | 1 | 1 | 1 | 1 |
| 1976 | P75_C3 | NA | 0 | 1 | 1 | 1 | 1 |
| 2076 | P75_D3 | NA | 0 | 1 | 1 | 1 | 1 |
| 21115 | P75_E3 | NA | 0 | 1 | 1 | 1 | 1 |
| 2376 | P75_G3 | NA | 0 | 1 | 1 | 1 | 1 |
| 2876 | P75_D4 | NA | 0 | 1 | 1 | 1 | 1 |
| 2976 | P75_E4 | NA | 0 | 1 | 1 | 1 | 1 |
| 3076 | P75_F4 | NA | 0 | 1 | 1 | 1 | 1 |
| 31115 | P75_G4 | NA | 0 | 1 | 1 | 1 | 1 |
| 3284 | P75_H4 | NA | 0 | 1 | 1 | 1 | 1 |
| 3376 | P75_A5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3476 | P75_B5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3676 | P75_D5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3776 | P75_E5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3876 | P75_F5 | NA | 0 | 1 | 1 | 1 | 1 |
| 3976 | P75_G5 | NA | 0 | 1 | 1 | 1 | 1 |
| 4076 | P75_H5 | NA | 0 | 1 | 1 | 1 | 1 |
| 41115 | P75_A6 | NA | 0 | 1 | 1 | 1 | 1 |
| 4284 | P75_B6 | NA | 0 | 1 | 1 | 1 | 1 |
| 4476 | P75_D6 | NA | 0 | 1 | 1 | 1 | 1 |
| 4576 | P75_E6 | NA | 0 | 1 | 1 | 1 | 1 |
| 4676 | P75_F6 | NA | 0 | 1 | 1 | 1 | 1 |
| 4776 | P75_G6 | NA | 0 | 1 | 1 | 1 | 1 |
| 5076 | P75_B7 | NA | 0 | 1 | 1 | 1 | 1 |
| 5476 | P75_F7 | NA | 0 | 1 | 1 | 1 | 1 |
| 5676 | P75_H7 | NA | 0 | 1 | 1 | 1 | 1 |
| 5776 | P75_A8 | NA | 0 | 1 | 1 | 1 | 1 |
| 5876 | P75_B8 | NA | 0 | 1 | 1 | 1 | 1 |
| 5976 | P75_C8 | NA | 0 | 1 | 1 | 1 | 1 |
| 6076 | P75_D8 | NA | 0 | 1 | 1 | 1 | 1 |
| 6284 | P75_F8 | NA | 0 | 1 | 1 | 1 | 1 |
| 6376 | P75_G8 | NA | 0 | 1 | 1 | 1 | 1 |
| 6576 | P75_A9 | NA | 0 | 1 | 1 | 0 | 1 |
| 6676 | P75_B9 | NA | 0 | 1 | 1 | 1 | 1 |
| 6776 | P75_C9 | NA | 0 | 1 | 1 | 1 | 1 |
| 6876 | P75_D9 | NA | 0 | 1 | 1 | 1 | 1 |
| 7076 | P75_F9 | NA | 0 | 1 | 1 | 1 | 1 |
| 71115 | P75_G9 | NA | 0 | 1 | 1 | 1 | 1 |
| 7284 | P75_H9 | NA | 0 | 1 | 1 | 1 | 1 |
| 7476 | P75_B10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7576 | P75_C10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7676 | P75_D10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7776 | P75_E10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7876 | P75_F10 | NA | 0 | 1 | 1 | 1 | 1 |
| 7976 | P75_G10 | NA | 0 | 1 | 1 | 1 | 1 |
| 8284 | P75_B11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8376 | P75_C11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8476 | P75_D11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8576 | P75_E11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8676 | P75_F11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8776 | P75_G11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8876 | P75_H11 | NA | 0 | 1 | 1 | 1 | 1 |
| 8976 | P75_A12 | NA | 0 | 1 | 1 | 1 | 1 |
| 91101 | P75_C12 | NA | 0 | 1 | 1 | 1 | 1 |
| 9277 | P75_D12 | NA | 0 | 1 | 1 | 1 | 1 |
| 9476 | P75_F12 | NA | 0 | 1 | 1 | 1 | 1 |
Year 1: Summarized sequences (ASVs) identified per sample
| monoclonal | oligoclonal | PositiveControl | NegativeControl | |
|---|---|---|---|---|
| LC=0 and HC=0 | 391 | 93 | 53 | 127 |
| LC=0 and HC=1 | 22 | 4 | 0 | 0 |
| LC=1 and HC=0 | 282 | 26 | 3 | 1 |
| LC=0 and HC>1 | 1 | 30 | 0 | 0 |
| LC>1 and HC=0 | 64 | 36 | 0 | 0 |
| LC=1 and HC=1 | 2184 | 96 | 27 | 0 |
| LC>1 or HC>1 (both > 0) | 894 | 1306 | 23 | 1 |
Year 2: Summarized sequences (ASVs) identified per sample
| monoclonal | oligoclonal | PositiveControl | NegativeControl | |
|---|---|---|---|---|
| LC=0 and HC=0 | 244 | 36 | 6 | 41 |
| LC=0 and HC=1 | 13 | 2 | 0 | 0 |
| LC=1 and HC=0 | 70 | 3 | 0 | 3 |
| LC=0 and HC>1 | 0 | 0 | 0 | 0 |
| LC>1 and HC=0 | 21 | 2 | 0 | 0 |
| LC=1 and HC=1 | 732 | 174 | 4 | 1 |
| LC>1 or HC>1 (both > 0) | 291 | 78 | 6 | 1 |
What is the concordance between ANARCI prediction and primers trimmed?
Rows indicate the expected prediction based on which primers were identified, columns are ANARCI prediction. An ANARCI prediction of "-" means that no prediction was made.
|
|
H | K | L | |
|---|---|---|---|---|
| HC | 294 | 8935 | 1 | 0 |
| LC | 632 | 0 | 11167 | 668 |
Basic statistics
Reads per plate:
Overall stats
| plate | RawReads | demuxcounts | PCT.DMX | hcReads | lcReads | aberrantReads | PCT.Ab | hcFewReads | lcFewReads | hcFewOverlapped | lcFewOverlapped | hcReported | lcReported | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | TRIMMER0002_P2 | 571113 | 304026 | 53.23395 | 75408 | 228618 | 80088 | 35.03136 | 2 | 2 | 2 | 2 | 87 | 88 |
| 110 | TRIMMER0003_P3 | 1684305 | 747844 | 44.40075 | 197906 | 549938 | 221821 | 40.33564 | 9 | 9 | 9 | 9 | 87 | 87 |
| 112 | TRIMMER0004.2_P4 | 1682719 | 669942 | 39.81306 | 202882 | 467060 | 162326 | 34.75485 | 7 | 6 | 8 | 6 | 82 | 86 |
| 114 | TRIMMER0005_P5 | 1908371 | 737908 | 38.66690 | 248280 | 489628 | 179969 | 36.75627 | 10 | 7 | 10 | 8 | 77 | 80 |
| 116 | TRIMMER0006_P6 | 1443039 | 585913 | 40.60271 | 187717 | 398196 | 114406 | 28.73108 | 28 | 24 | 28 | 26 | 62 | 62 |
| 118 | TRIMMER0007_P7 | 1168633 | 410921 | 35.16254 | 152961 | 257960 | 95433 | 36.99527 | 8 | 6 | 10 | 6 | 80 | 84 |
| 120 | TRIMMER0010_P10R | 661554 | 154918 | 23.41729 | 788 | 154130 | 61403 | 39.83845 | 68 | 60 | 76 | 60 | 2 | 32 |
| 127 | TRIMMER0013_P28 | 1637909 | 586930 | 35.83410 | 167911 | 419019 | 172283 | 41.11580 | 0 | 0 | 0 | 0 | 70 | 90 |
| 129 | TRIMMER0014_P29 | 1163087 | 257317 | 22.12362 | 88190 | 169127 | 51526 | 30.46586 | 3 | 1 | 4 | 1 | 78 | 89 |
| 130 | TRIMMER0015_P30 | 860187 | 187889 | 21.84281 | 62289 | 125600 | 39238 | 31.24045 | 3 | 2 | 5 | 2 | 76 | 82 |
| 140 | TRIMMER0016_P31 | 1492272 | 373133 | 25.00436 | 123295 | 249838 | 88118 | 35.27005 | 0 | 0 | 0 | 0 | 78 | 85 |
| 150 | TRIMMER0017_P45 | 1637424 | 351926 | 21.49266 | 93414 | 258512 | 69892 | 27.03627 | 1 | 1 | 1 | 2 | 79 | 89 |
| 160 | TRIMMER0018_P8 | 1359951 | 542317 | 39.87769 | 172102 | 370215 | 141228 | 38.14756 | 2 | 0 | 2 | 1 | 80 | 85 |
| 170 | TRIMMER0019_P9 | 1251720 | 540508 | 43.18122 | 149384 | 391124 | 152259 | 38.92858 | 5 | 4 | 8 | 4 | 68 | 87 |
| 180 | TRIMMER0020_P10 | 1382743 | 508309 | 36.76092 | 92486 | 415823 | 151553 | 36.44652 | 10 | 2 | 14 | 3 | 48 | 86 |
| 190 | TRIMMER0021_P11 | 1387628 | 559219 | 40.30035 | 129104 | 430115 | 160797 | 37.38465 | 9 | 5 | 10 | 5 | 64 | 83 |
| 1100 | TRIMMER0022_P12 | 1400058 | 600986 | 42.92579 | 200949 | 400037 | 102918 | 25.72712 | 3 | 2 | 3 | 3 | 86 | 92 |
| 1101 | TRIMMER0023_P13 | 1522181 | 653014 | 42.89989 | 146839 | 506175 | 229933 | 45.42559 | 3 | 2 | 4 | 2 | 68 | 89 |
| 1102 | TRIMMER0024_P14 | 1928710 | 837487 | 43.42213 | 222478 | 615009 | 223753 | 36.38207 | 9 | 4 | 12 | 5 | 52 | 70 |
| 1103 | TRIMMER0026_P16 | 1378979 | 557860 | 40.45457 | 143772 | 414088 | 127797 | 30.86228 | 6 | 2 | 6 | 4 | 81 | 88 |
| 1104 | TRIMMER0027_P33 | 870290 | 446766 | 51.33530 | 131748 | 315018 | 132525 | 42.06902 | 8 | 3 | 16 | 3 | 63 | 80 |
| 1105 | TRIMMER0028_P34 | 766313 | 401893 | 52.44502 | 163282 | 238611 | 78587 | 32.93520 | 5 | 1 | 13 | 2 | 77 | 89 |
| 1106 | TRIMMER0029_P35 | 865332 | 425476 | 49.16911 | 154320 | 271156 | 101072 | 37.27448 | 2 | 1 | 8 | 1 | 78 | 81 |
| 1107 | TRIMMER0030_P36 | 1198844 | 591499 | 49.33911 | 209841 | 381658 | 122177 | 32.01217 | 5 | 1 | 7 | 1 | 79 | 89 |
| 1108 | TRIMMER0031_P15 | 846682 | 379940 | 44.87399 | 131532 | 248408 | 80270 | 32.31377 | 4 | 2 | 9 | 2 | 62 | 76 |
| 1109 | TRIMMER0033_P17 | 882659 | 432611 | 49.01225 | 165954 | 266657 | 115077 | 43.15544 | 5 | 2 | 6 | 2 | 75 | 83 |
| 1130 | TRIMMER0034_P18 | 1217682 | 594387 | 48.81299 | 253379 | 341008 | 90832 | 26.63633 | 1 | 2 | 2 | 2 | 78 | 88 |
| 1132 | TRIMMER0035_P19 | 1087896 | 534267 | 49.11012 | 222215 | 312052 | 89658 | 28.73175 | 3 | 1 | 4 | 2 | 84 | 88 |
| 1134 | TRIMMER0036_P20 | 857227 | 439583 | 51.27965 | 182702 | 256881 | 91492 | 35.61649 | 7 | 6 | 8 | 6 | 78 | 85 |
| 1136 | TRIMMER0037_P21 | 906420 | 479596 | 52.91101 | 157886 | 321710 | 106919 | 33.23459 | 5 | 3 | 12 | 3 | 68 | 85 |
| 1138 | TRIMMER0038_P22 | 961787 | 482320 | 50.14832 | 148460 | 333860 | 131848 | 39.49200 | 3 | 3 | 5 | 3 | 83 | 92 |
| 1140 | TRIMMER0039_P23 | 1012149 | 505116 | 49.90530 | 190468 | 314648 | 129703 | 41.22162 | 3 | 3 | 3 | 3 | 86 | 93 |
| 1142 | TRIMMER0040_P24 | 713687 | 344704 | 48.29904 | 155448 | 189256 | 72400 | 38.25506 | 3 | 3 | 5 | 3 | 88 | 89 |
| 1144 | TRIMMER0041_P25 | 878452 | 410411 | 46.71980 | 163635 | 246776 | 80004 | 32.41968 | 3 | 2 | 3 | 2 | 90 | 90 |
| 1146 | TRIMMER0042_P26 | 817238 | 385514 | 47.17279 | 149642 | 235872 | 61152 | 25.92593 | 5 | 1 | 6 | 3 | 79 | 83 |
| 1148 | TRIMMER0043_P27 | 941046 | 425255 | 45.18961 | 158740 | 266515 | 99160 | 37.20616 | 6 | 2 | 7 | 2 | 77 | 80 |
| 1150 | TRIMMER0044_P37 | 1195904 | 783237 | 65.49330 | 262771 | 520466 | 210772 | 40.49679 | 3 | 2 | 5 | 2 | 76 | 93 |
| 1152 | TRIMMER0045_P38 | 1109354 | 703337 | 63.40059 | 264957 | 438380 | 180199 | 41.10566 | 6 | 5 | 6 | 5 | 77 | 91 |
| 1154 | TRIMMER0046_P39 | 966125 | 626457 | 64.84223 | 195548 | 430909 | 210318 | 48.80798 | 5 | 5 | 6 | 5 | 70 | 91 |
| 1156 | TRIMMER0047_P40 | 1182635 | 739123 | 62.49798 | 183035 | 556088 | 236402 | 42.51162 | 6 | 2 | 12 | 2 | 63 | 89 |
| 1158 | TRIMMER0048_P41 | 1243060 | 825608 | 66.41739 | 304705 | 520903 | 221330 | 42.48968 | 7 | 5 | 9 | 5 | 77 | 90 |
| 1160 | TRIMMER0049_P42 | 1042908 | 705715 | 67.66800 | 245921 | 459794 | 205840 | 44.76787 | 4 | 3 | 9 | 4 | 77 | 88 |
| 1162 | TRIMMER0050_P43 | 734473 | 495020 | 67.39798 | 177546 | 317474 | 121678 | 38.32692 | 4 | 3 | 4 | 3 | 90 | 92 |
| 1164 | TRIMMER0051_P44 | 922254 | 618484 | 67.06222 | 219444 | 399040 | 154134 | 38.62620 | 3 | 2 | 7 | 2 | 87 | 93 |
| 1166 | TRIMMER0052_P46 | 834525 | 559073 | 66.99296 | 159049 | 400024 | 148349 | 37.08502 | 6 | 4 | 6 | 4 | 89 | 92 |
| 1168 | TRIMMER0053_P47 | 966609 | 648902 | 67.13180 | 208894 | 440008 | 157572 | 35.81117 | 6 | 3 | 7 | 3 | 89 | 90 |
| 1170 | TRIMMER0054_P48 | 1051379 | 707912 | 67.33176 | 268424 | 439488 | 117992 | 26.84760 | 5 | 5 | 5 | 5 | 90 | 91 |
| 1172 | TRIMMER0055_P49 | 934382 | 635271 | 67.98836 | 246892 | 388379 | 130664 | 33.64343 | 5 | 4 | 5 | 4 | 88 | 92 |
| 1174 | TRIMMER0056_P50 | 818736 | 550103 | 67.18930 | 171867 | 378236 | 152996 | 40.44988 | 4 | 3 | 7 | 3 | 83 | 91 |
| 1176 | TRIMMER0057_P51 | 979265 | 643470 | 65.70949 | 257470 | 386000 | 116605 | 30.20855 | 4 | 3 | 4 | 3 | 92 | 92 |
| 1178 | TRIMMER0058_P52 | 1048688 | 680023 | 64.84512 | 216639 | 463384 | 167697 | 36.18964 | 2 | 1 | 2 | 1 | 92 | 93 |
| 1180 | TRIMMER0059_P54 | 864904 | 550011 | 63.59214 | 192296 | 357715 | 146336 | 40.90854 | 2 | 1 | 2 | 1 | 92 | 95 |
| 1182 | TRIMMER0060_P55 | 871030 | 585848 | 67.25922 | 214410 | 371438 | 147377 | 39.67742 | 3 | 3 | 4 | 3 | 91 | 93 |
| 1184 | TRIMMER0061_P56 | 1000302 | 673345 | 67.31417 | 219625 | 453720 | 167602 | 36.93952 | 3 | 3 | 3 | 3 | 91 | 93 |
| 1186 | TRIMMER0062_P57 | 1404759 | 926346 | 65.94341 | 383917 | 542429 | 193398 | 35.65407 | 4 | 3 | 4 | 4 | 89 | 92 |
| 1188 | TRIMMER0063_P58 | 848043 | 517933 | 61.07391 | 206040 | 311893 | 93647 | 30.02536 | 5 | 2 | 6 | 3 | 90 | 91 |
| 1190 | TRIMMER0064_P59 | 1279777 | 831088 | 64.94006 | 267019 | 564069 | 216245 | 38.33662 | 5 | 2 | 5 | 2 | 89 | 93 |
| 1192 | TRIMMER0065_P60 | 1112888 | 723069 | 64.97231 | 221748 | 501321 | 219864 | 43.85693 | 3 | 3 | 4 | 3 | 83 | 93 |
| 1194 | TRIMMER0066_P65 | 1130112 | 722677 | 63.94738 | 300747 | 421930 | 159780 | 37.86884 | 6 | 4 | 8 | 4 | 81 | 85 |
| 1196 | TRIMMER0067_P66 | 761515 | 491951 | 64.60162 | 213764 | 278187 | 106973 | 38.45363 | 6 | 6 | 6 | 6 | 90 | 90 |
| 1198 | TRIMMER0068_P1R | 367088 | 124332 | 33.86981 | 41672 | 82660 | 32744 | 39.61287 | 9 | 5 | 10 | 5 | 85 | 91 |
| 1200 | TRIMMER0069_PDP1 | 2337556 | 1507646 | 64.49668 | 209881 | 1297765 | 912057 | 70.27906 | 18 | 9 | 18 | 9 | 47 | 55 |
| 1201 | TRIMMER0070_PDP2 | 2498629 | 1664750 | 66.62654 | 328191 | 1336559 | 878067 | 65.69609 | 6 | 1 | 8 | 2 | 53 | 70 |
| 1202 | TRIMMER0073_PS1 | 2748584 | 1806289 | 65.71707 | 676631 | 1129658 | 495904 | 43.89860 | 7 | 6 | 8 | 6 | 81 | 88 |
| 1203 | TRIMMER0074_PS2 | 3869822 | 2505308 | 64.73962 | 791827 | 1713481 | 694712 | 40.54390 | 9 | 7 | 9 | 8 | 82 | 86 |
| 1204 | TRIMMER0075_PS3 | 3621378 | 2230240 | 61.58540 | 891079 | 1339161 | 495193 | 36.97785 | 3 | 1 | 3 | 1 | 89 | 92 |
| 1205 | TRIMMER0077_P32R | 2021192 | 1265368 | 62.60504 | 390561 | 874807 | 271337 | 31.01678 | 4 | 0 | 4 | 0 | 78 | 89 |
| 1206 | TRIMMER0078_P67 | 1770696 | 1184371 | 66.88731 | 446632 | 737739 | 262418 | 35.57057 | 4 | 3 | 4 | 3 | 91 | 91 |
| 1207 | TRIMMER0079_P68 | 1896521 | 1262527 | 66.57068 | 504750 | 757777 | 288438 | 38.06370 | 5 | 4 | 5 | 4 | 86 | 90 |
| 1208 | TRIMMER0080_P69 | 1973427 | 1258260 | 63.76015 | 483539 | 774721 | 357605 | 46.15920 | 6 | 3 | 7 | 5 | 84 | 87 |
| 1209 | TRIMMER0076_P6R | 1372694 | 881211 | 64.19573 | 323472 | 557739 | 154176 | 27.64304 | 4 | 3 | 4 | 3 | 81 | 84 |
| 1273 | TRIMMER0081_P70 | 2005190 | 1310164 | 65.33865 | 497153 | 813011 | 301520 | 37.08683 | 2 | 1 | 2 | 1 | 88 | 91 |
| 1275 | TRIMMER0082_P71 | 1534823 | 998923 | 65.08392 | 414981 | 583942 | 242670 | 41.55721 | 8 | 3 | 8 | 3 | 80 | 85 |
| 1277 | TRIMMER0083_P72 | 1549641 | 939475 | 60.62533 | 413077 | 526398 | 188531 | 35.81530 | 4 | 3 | 6 | 3 | 83 | 88 |
| 1279 | TRIMMER0084_P73 | 593252 | 370094 | 62.38394 | 95406 | 274688 | 128749 | 46.87100 | 5 | 3 | 5 | 3 | 83 | 88 |
| 1281 | TRIMMER0085_P74 | 489362 | 297965 | 60.88846 | 103832 | 194133 | 66371 | 34.18842 | 10 | 11 | 10 | 11 | 83 | 84 |
| 1283 | TRIMMER0086_P75 | 386039 | 232809 | 60.30712 | 79573 | 153236 | 66373 | 43.31423 | 6 | 6 | 9 | 6 | 87 | 87 |
Samples that didn't produce an ASV in one or both chains:
Variance in reads by SMARTindex (sample) across plates
Values on the Y-axis are per-primer pair and calculated like:
100 * (number of reads with correct primer sequence)/(total number of reads for the plate).
The better the pooling and more consistently samples performed within plate, the less variance in percent reads there should be.
Optimal y-value should be ~ 0.5 because there are 96*2 barcode pairs (100/192).
The values for LC include the aberrant reads.
Percentage of reads by Primer+Index
Note that the denominator is total reads per plate. 
Variance in total reads by Primer+Index
Variance in percent reads with primer by Plate
Percent of reads with Primer+Index that could be overlapped
Percent overlap should be ~100 because insert lengths are short enough that all on-target amplicons should overlap unless read quality was low. There should also be very low plate-to-plate or sample-to-sample variance in overlap unless read quality was low, there were a large number of primer dimers or something else went wrong.
ASVs supported by 10 or more reads
Number of HC ASVs with support from 10 or more reads:
In plates containing monoclonal samples, each sample should contain one heavy chain and two light chain sequences. Plates that contain non-monoclonal (parent) samples will have multiple sequences. The values reported here represent pre-filtered ASV counts for ASVs with more than 10 supporting reads.
Number of HC ASVs reported:
This plot shows the distribution of number of HC ASVs reported after filtering per plate.
Number of LC ASVs with support from 10 or more reads:
In plates containing monoclonal samples, each sample should contain one heavy chain and two light chain sequences. Plates that contain non-monoclonal (parent) samples will have multiple sequences. The values reported here represent pre-filtered ASV counts for ASVs with more than 10 supporting reads.
Number of LC ASVs reported:
This plot shows the distribution of number of LC ASVs reported after filtering per plate.
Other plots..
Light chain
Count of overlapped reads, samples with < 10 reads are Red, samples with 10-50 reads a yellow, samples with > 50 reads are green. 
Heavy Chain
Count of overlapped reads, samples with < 10 reads are Red, samples with 10-50 reads a yellow, samples with > 50 reads are green. 
Duplicate ASVs
See TSV file for duplicate results.