NOTE: only the plates in the following list are included in this report.

## TRIMMER0002_P2
## TRIMMER0003_P3
## TRIMMER0004.2_P4
## TRIMMER0005_P5
## TRIMMER0006_P6
## TRIMMER0007_P7
## TRIMMER0010_P10R
## TRIMMER0013_P28
## TRIMMER0014_P29
## TRIMMER0015_P30
## TRIMMER0016_P31
## TRIMMER0017_P45
## TRIMMER0018_P8
## TRIMMER0019_P9
## TRIMMER0020_P10
## TRIMMER0021_P11
## TRIMMER0022_P12
## TRIMMER0023_P13
## TRIMMER0024_P14
## TRIMMER0026_P16
## TRIMMER0027_P33
## TRIMMER0028_P34
## TRIMMER0029_P35
## TRIMMER0030_P36
## TRIMMER0031_P15
## TRIMMER0033_P17
## TRIMMER0034_P18
## TRIMMER0035_P19
## TRIMMER0036_P20
## TRIMMER0037_P21
## TRIMMER0038_P22
## TRIMMER0039_P23
## TRIMMER0040_P24
## TRIMMER0041_P25
## TRIMMER0042_P26
## TRIMMER0043_P27
## TRIMMER0044_P37
## TRIMMER0045_P38
## TRIMMER0046_P39
## TRIMMER0047_P40
## TRIMMER0048_P41
## TRIMMER0049_P42
## TRIMMER0050_P43
## TRIMMER0051_P44
## TRIMMER0052_P46
## TRIMMER0053_P47
## TRIMMER0054_P48
## TRIMMER0055_P49
## TRIMMER0056_P50
## TRIMMER0057_P51
## TRIMMER0058_P52
## TRIMMER0059_P54
## TRIMMER0060_P55
## TRIMMER0061_P56
## TRIMMER0062_P57
## TRIMMER0063_P58
## TRIMMER0064_P59
## TRIMMER0065_P60
## TRIMMER0066_P65
## TRIMMER0067_P66
## TRIMMER0068_P1R
## TRIMMER0069_PDP1
## TRIMMER0070_PDP2
## TRIMMER0073_PS1
## TRIMMER0074_PS2
## TRIMMER0075_PS3
## TRIMMER0077_P32R
## TRIMMER0078_P67
## TRIMMER0079_P68
## TRIMMER0080_P69
## TRIMMER0076_P6R
## TRIMMER0081_P70
## TRIMMER0082_P71
## TRIMMER0083_P72
## TRIMMER0084_P73
## TRIMMER0085_P74
## TRIMMER0086_P75
## ERROR: Annotated results have Sample_Names that are missing from metadata file.

Summary statistics by funding period

Funding periods Year1 = July 1 2019 - June 30 2020 Year2 = July 1 2020 - June 30, 2021 Year3 = July 1 2021 - June 30, 2022

Within each of the above date ranges, report a count of: 1) total number of monoclonal hybridomas sequenced 2) how many of these yielded only one heavy and one light chain sequence (as annotated by ANARCI) 3) how many of these had more than one heavy or light chain (or both) 4) total number of oligoclonal/parent hybridomas sequenced

Total plates sequenced per funding year:

Var1 Freq
Year1 59
Year2 18

Total number of monoclonal, oligoclonal, and control samples sequenced per funding year.

Year1 Year2
monoclonal 3838 1371
oligoclonal 1591 295
PositiveControl 106 16
NegativeControl 129 46

Summarized sequences (ASVs) identified per sample for all funding years.

Values are number of samples that fall within each category.

Note that these categories were generated using the number of sequences identified in each sample where ANARCI could categorize the resulting sequence and predict domains.

monoclonal oligoclonal PositiveControl NegativeControl
LC=0 and HC=0 635 129 59 168
LC=0 and HC=1 35 6 0 0
LC=1 and HC=0 352 29 3 4
LC=0 and HC>1 1 30 0 0
LC>1 and HC=0 85 38 0 0
LC=1 and HC=1 2916 270 31 1
LC>1 or HC>1 (both > 0) 1185 1384 29 2

Summarized sequences by Sample Category

Sample Categories include:

  1. NeuroMab mAbs
  2. Non-NeuroMab mAbs
  3. NeuroMab Alternative Subclones
  4. Lead oligoclonal Abs

Monoclonal samples:

0 1 2 3 4
LC=0 and HC=0 0 40 495 100 0
LC=0 and HC=1 0 4 28 3 0
LC=0 and HC>1 0 0 1 0 0
LC=1 and HC=0 1 12 297 42 0
LC=1 and HC=1 5 404 1818 688 1
LC>1 and HC=0 0 1 65 19 0
LC>1 or HC>1 (both > 0) 36 112 714 323 0

Oligoclonal samples:

0 4
LC=0 and HC=0 31 98
LC=0 and HC=1 2 4
LC=0 and HC>1 0 30
LC=1 and HC=0 2 27
LC=1 and HC=1 173 97
LC>1 and HC=0 2 36
LC>1 or HC>1 (both > 0) 79 1305

Usable as is oligoclonal samples: These are samples that were originally flagged as likely to be oligoclonal, but produced only a single HC and LC, suggesting that they are probably monoclonal.

sample_name trimmer_id Category LCs.Annotated HCs.Annotated LC.K.Called HC.H.Called
350 P45_C1 L118/28 4 1 1 1 1
1411 P45_F2 L118/73 4 1 1 1 1
2811 P45_D4 L118/48 4 1 1 1 1
3411 P45_B5 L118/20 4 1 1 1 1
5811 P45_B8 L118/23 4 1 1 1 1
6115 P45_E8 L118/68 4 1 1 1 1
8511 P45_E11 L118/70 4 1 1 1 1
2942 P43_E4 L21/14 4 1 1 1 1
3842 P43_F5 N486/7 4 1 1 1 1
4442 P43_D6 N34/1 4 1 1 1 1
5244 P43_D7 N34/17 4 1 1 1 1
6842 P43_D9 N34/31 4 1 1 1 1
4164 P44_D1 K28/9 4 1 1 1 1
1543 P44_G2 N279A/16 4 1 1 1 1
1743 P44_A3 K28/92 4 1 1 1 1
1843 P44_B3 K28/33 4 1 1 1 1
3943 P44_G5 N279A/40 4 1 1 1 1
4245 P44_B6 K28/30 4 1 1 1 1
6543 P44_A9 K28/45 4 1 1 1 1
6643 P44_B9 K28/26 4 1 1 1 1
6843 P44_D9 K28/1 4 1 1 0 1
7165 P44_G9 N327/45 4 1 1 1 1
8343 P44_C11 K28/11 4 1 1 1 1
8643 P44_F11 L114/22 4 1 1 1 1
5845 P47_B8 L124/47 4 1 1 0 1
3347 P49_A5 K20/58 4 1 1 1 1
4173 P49_A6 K20/59 4 1 1 1 1
4947 P49_A7 K20/74 4 1 1 1 1
5747 P49_A8 K20/82 4 1 1 1 1
6547 P49_A9 K20/83 4 1 1 1 1
6847 P49_D9 L127/74 4 1 1 1 1
7249 P49_H9 K20/86 4 1 1 1 1
8947 P49_A12 K20/91 4 1 1 1 1
7174 P50_G1 N437/23 4 1 1 1 1
1175 P50_C2 N275/31 4 1 1 1 1
2648 P50_B4 N275/12 4 1 1 1 1
2948 P50_E4 N69/17 4 1 1 1 1
3548 P50_C5 N275/35 4 1 1 1 1
4048 P50_H5 L107/68 4 1 1 1 1
4448 P50_D6 N269/30 4 1 1 1 1
5948 P50_C8 N275/38 4 1 1 0 1
6348 P50_G8 N437/81 4 1 1 1 1
6448 P50_H8 K36/5 4 1 1 1 1
6648 P50_B9 N275/20 4 1 1 1 1
6748 P50_C9 N275/41 4 1 1 1 1
7250 P50_H9 L107/86 4 1 1 1 1
7348 P50_A10 N275/3 4 1 1 1 1
7648 P50_D10 N282A/23 4 1 1 1 1
8250 P50_B11 N275/25 4 1 1 1 1
9448 P50_F12 N437/9 4 1 1 1 1
7182 P55_G1 L117/85 4 1 1 1 1
6652 P55_B9 L117/19 4 1 1 1 1
6852 P55_D9 L117/45 4 1 1 1 1
8952 P55_A12 L113/120 4 1 1 1 1
6853 P56_D9 L119/34 4 1 1 1 1
7453 P56_B10 L117/131 4 1 1 1 1
8185 P56_A11 L117/9 4 1 1 1 1
1454 P57_F2 L120/46 4 1 1 1 1
2187 P57_E3 L120/35 4 1 1 1 1
2454 P57_H3 L120/71 4 1 1 1 1
2654 P57_B4 L119/144 4 1 1 1 1
2854 P57_D4 L120/22 4 1 1 1 1
2954 P57_E4 L120/36 4 1 1 1 1
3654 P57_D5 L120/23 4 1 1 1 1
3854 P57_F5 L120/49 4 1 1 1 1
4054 P57_H5 L120/74 4 1 1 1 1
4354 P57_C6 L120/11 4 1 1 1 1
4854 P57_H6 L120/75 4 1 1 1 1
5354 P57_E7 L120/39 4 1 1 1 1
5454 P57_F7 L120/51 4 1 1 1 1
6354 P57_G8 L120/63 4 1 1 1 1
8454 P57_D11 L120/31 4 1 1 1 1
9255 P57_D12 L120/32 4 1 1 1 1
9354 P57_E12 L120/44 4 1 1 1 1
3188 P58_C1 L120/101 4 1 1 1 1
1055 P58_B2 L120/88 4 1 1 1 1
2755 P58_C4 L120/104 4 1 1 1 1
3055 P58_F4 L120/140 4 1 1 1 1
3455 P58_B5 L120/93 4 1 1 1 1
3655 P58_D5 L120/117 4 1 1 1 1
4257 P58_B6 L120/94 4 1 1 1 1
4655 P58_F6 L120/142 4 1 1 1 1
6055 P58_D8 L120/120 4 1 1 1 1
6555 P58_A9 L120/82 4 1 1 1 1
9160 P58_C12 L120/112 4 1 1 1 1
6190 P59_F1 L122/23 4 1 1 1 1
2258 P59_F3 L122/26 4 1 1 1 1
6056 P59_D8 L121/144 4 1 1 1 1
1193 P60_C2 L122/67 4 1 1 1 1
3259 P60_H4 L122/134 4 1 1 1 1
4857 P60_H6 L122/136 4 1 1 1 1
5193 P60_C7 L122/74 4 1 1 1 1
5957 P60_C8 L122/75 4 1 1 1 1
7457 P60_B10 L122/63 4 1 1 1 1
7195 P65_G9 N29/1 4 1 1 1 1
9458 P65_F12 N34/35 4 1 1 1 1
9174 PDP2_C12 L119/87 4 1 1 1 1
1279 P73_A1 NA 0 1 1 1 1
2279 P73_B1 NA 0 1 1 1 1
3279 P73_C1 NA 0 1 1 1 1
7279 P73_G1 NA 0 1 1 0 1
8279 P73_H1 NA 0 1 1 1 1
9197 P73_A2 NA 0 1 1 1 1
1074 P73_B2 NA 0 1 1 1 1
11113 P73_C2 NA 0 1 1 1 1
1474 P73_F2 NA 0 1 1 1 1
1574 P73_G2 NA 0 1 1 0 1
1674 P73_H2 NA 0 1 1 1 1
1774 P73_A3 NA 0 1 1 1 1
1874 P73_B3 NA 0 1 1 1 1
1974 P73_C3 NA 0 1 1 1 1
2280 P73_F3 NA 0 1 1 1 1
2374 P73_G3 NA 0 1 1 1 1
2574 P73_A4 NA 0 1 1 1 1
2674 P73_B4 NA 0 1 1 1 1
2874 P73_D4 NA 0 1 1 1 1
3280 P73_H4 NA 0 1 1 1 1
3374 P73_A5 NA 0 1 1 1 1
3474 P73_B5 NA 0 1 1 1 1
3574 P73_C5 NA 0 1 1 1 1
3774 P73_E5 NA 0 1 1 1 1
4280 P73_B6 NA 0 1 1 1 1
4374 P73_C6 NA 0 1 1 1 1
4474 P73_D6 NA 0 1 1 1 1
5074 P73_B7 NA 0 1 1 1 1
51113 P73_C7 NA 0 1 1 1 1
5280 P73_D7 NA 0 1 1 1 1
5374 P73_E7 NA 0 1 1 1 1
5474 P73_F7 NA 0 1 1 1 1
5674 P73_H7 NA 0 1 1 1 1
5974 P73_C8 NA 0 1 1 1 1
6280 P73_F8 NA 0 1 1 1 1
6374 P73_G8 NA 0 1 1 1 1
6674 P73_B9 NA 0 1 1 1 1
6774 P73_C9 NA 0 1 1 1 1
71113 P73_G9 NA 0 1 1 0 1
7474 P73_B10 NA 0 1 1 1 1
7674 P73_D10 NA 0 1 1 1 1
8074 P73_H10 NA 0 1 1 1 1
8374 P73_C11 NA 0 1 1 1 1
8674 P73_F11 NA 0 1 1 1 1
8874 P73_H11 NA 0 1 1 1 1
9198 P73_C12 NA 0 1 1 1 1
9474 P73_F12 NA 0 1 1 1 1
2281 P74_B1 NA 0 1 1 1 1
4281 P74_D1 NA 0 1 1 1 1
5281 P74_E1 NA 0 1 1 1 1
6281 P74_F1 NA 0 1 1 1 1
8281 P74_H1 NA 0 1 1 1 1
9199 P74_A2 NA 0 1 1 1 1
1075 P74_B2 NA 0 1 1 1 1
1475 P74_F2 NA 0 1 1 1 1
1775 P74_A3 NA 0 1 1 1 1
1875 P74_B3 NA 0 1 1 1 1
1975 P74_C3 NA 0 1 1 1 1
2075 P74_D3 NA 0 1 1 1 1
2282 P74_F3 NA 0 1 1 1 1
2375 P74_G3 NA 0 1 1 1 1
2575 P74_A4 NA 0 1 1 1 1
2675 P74_B4 NA 0 1 1 1 1
2775 P74_C4 NA 0 1 1 1 1
2875 P74_D4 NA 0 1 1 1 1
3075 P74_F4 NA 0 1 1 1 1
3375 P74_A5 NA 0 1 1 1 1
3475 P74_B5 NA 0 1 1 1 1
3675 P74_D5 NA 0 1 1 1 1
3775 P74_E5 NA 0 1 1 1 1
3975 P74_G5 NA 0 1 1 1 1
41114 P74_A6 NA 0 1 1 1 1
4282 P74_B6 NA 0 1 1 1 1
4475 P74_D6 NA 0 1 1 1 1
4675 P74_F6 NA 0 1 1 1 1
5075 P74_B7 NA 0 1 1 1 1
5282 P74_D7 NA 0 1 1 1 1
5475 P74_F7 NA 0 1 1 0 1
5775 P74_A8 NA 0 1 1 1 1
5875 P74_B8 NA 0 1 1 1 1
5975 P74_C8 NA 0 1 1 1 1
6075 P74_D8 NA 0 1 1 1 1
61114 P74_E8 NA 0 1 1 1 1
6282 P74_F8 NA 0 1 1 1 1
6675 P74_B9 NA 0 1 1 1 1
6775 P74_C9 NA 0 1 1 1 1
6875 P74_D9 NA 0 1 1 1 1
6975 P74_E9 NA 0 1 1 1 1
7075 P74_F9 NA 0 1 1 1 1
7375 P74_A10 NA 0 1 1 1 1
7475 P74_B10 NA 0 1 1 1 1
7575 P74_C10 NA 0 1 1 1 1
7675 P74_D10 NA 0 1 1 1 1
7775 P74_E10 NA 0 1 1 1 1
7875 P74_F10 NA 0 1 1 1 1
7975 P74_G10 NA 0 1 1 1 1
81114 P74_A11 NA 0 1 1 1 1
8282 P74_B11 NA 0 1 1 1 1
8375 P74_C11 NA 0 1 1 1 1
8575 P74_E11 NA 0 1 1 1 1
8675 P74_F11 NA 0 1 1 1 1
8975 P74_A12 NA 0 1 1 1 1
9075 P74_B12 NA 0 1 1 1 1
91100 P74_C12 NA 0 1 1 1 1
9276 P74_D12 NA 0 1 1 1 1
9375 P74_E12 NA 0 1 1 1 1
9475 P74_F12 NA 0 1 1 1 1
9575 P74_G12 NA 0 1 1 1 1
2283 P75_B1 NA 0 1 1 1 1
4283 P75_D1 NA 0 1 1 1 1
5283 P75_E1 NA 0 1 1 1 1
7283 P75_G1 NA 0 1 1 1 1
8283 P75_H1 NA 0 1 1 1 1
11115 P75_C2 NA 0 1 1 1 1
1284 P75_D2 NA 0 1 1 1 1
1576 P75_G2 NA 0 1 1 1 1
1676 P75_H2 NA 0 1 1 1 1
1776 P75_A3 NA 0 1 1 1 1
1976 P75_C3 NA 0 1 1 1 1
2076 P75_D3 NA 0 1 1 1 1
21115 P75_E3 NA 0 1 1 1 1
2376 P75_G3 NA 0 1 1 1 1
2876 P75_D4 NA 0 1 1 1 1
2976 P75_E4 NA 0 1 1 1 1
3076 P75_F4 NA 0 1 1 1 1
31115 P75_G4 NA 0 1 1 1 1
3284 P75_H4 NA 0 1 1 1 1
3376 P75_A5 NA 0 1 1 1 1
3476 P75_B5 NA 0 1 1 1 1
3676 P75_D5 NA 0 1 1 1 1
3776 P75_E5 NA 0 1 1 1 1
3876 P75_F5 NA 0 1 1 1 1
3976 P75_G5 NA 0 1 1 1 1
4076 P75_H5 NA 0 1 1 1 1
41115 P75_A6 NA 0 1 1 1 1
4284 P75_B6 NA 0 1 1 1 1
4476 P75_D6 NA 0 1 1 1 1
4576 P75_E6 NA 0 1 1 1 1
4676 P75_F6 NA 0 1 1 1 1
4776 P75_G6 NA 0 1 1 1 1
5076 P75_B7 NA 0 1 1 1 1
5476 P75_F7 NA 0 1 1 1 1
5676 P75_H7 NA 0 1 1 1 1
5776 P75_A8 NA 0 1 1 1 1
5876 P75_B8 NA 0 1 1 1 1
5976 P75_C8 NA 0 1 1 1 1
6076 P75_D8 NA 0 1 1 1 1
6284 P75_F8 NA 0 1 1 1 1
6376 P75_G8 NA 0 1 1 1 1
6576 P75_A9 NA 0 1 1 0 1
6676 P75_B9 NA 0 1 1 1 1
6776 P75_C9 NA 0 1 1 1 1
6876 P75_D9 NA 0 1 1 1 1
7076 P75_F9 NA 0 1 1 1 1
71115 P75_G9 NA 0 1 1 1 1
7284 P75_H9 NA 0 1 1 1 1
7476 P75_B10 NA 0 1 1 1 1
7576 P75_C10 NA 0 1 1 1 1
7676 P75_D10 NA 0 1 1 1 1
7776 P75_E10 NA 0 1 1 1 1
7876 P75_F10 NA 0 1 1 1 1
7976 P75_G10 NA 0 1 1 1 1
8284 P75_B11 NA 0 1 1 1 1
8376 P75_C11 NA 0 1 1 1 1
8476 P75_D11 NA 0 1 1 1 1
8576 P75_E11 NA 0 1 1 1 1
8676 P75_F11 NA 0 1 1 1 1
8776 P75_G11 NA 0 1 1 1 1
8876 P75_H11 NA 0 1 1 1 1
8976 P75_A12 NA 0 1 1 1 1
91101 P75_C12 NA 0 1 1 1 1
9277 P75_D12 NA 0 1 1 1 1
9476 P75_F12 NA 0 1 1 1 1

Year 1: Summarized sequences (ASVs) identified per sample

monoclonal oligoclonal PositiveControl NegativeControl
LC=0 and HC=0 391 93 53 127
LC=0 and HC=1 22 4 0 0
LC=1 and HC=0 282 26 3 1
LC=0 and HC>1 1 30 0 0
LC>1 and HC=0 64 36 0 0
LC=1 and HC=1 2184 96 27 0
LC>1 or HC>1 (both > 0) 894 1306 23 1

Year 2: Summarized sequences (ASVs) identified per sample

monoclonal oligoclonal PositiveControl NegativeControl
LC=0 and HC=0 244 36 6 41
LC=0 and HC=1 13 2 0 0
LC=1 and HC=0 70 3 0 3
LC=0 and HC>1 0 0 0 0
LC>1 and HC=0 21 2 0 0
LC=1 and HC=1 732 174 4 1
LC>1 or HC>1 (both > 0) 291 78 6 1

What is the concordance between ANARCI prediction and primers trimmed?

Rows indicate the expected prediction based on which primers were identified, columns are ANARCI prediction. An ANARCI prediction of "-" means that no prediction was made.

H K L
HC 294 8935 1 0
LC 632 0 11167 668

Basic statistics

Reads per plate:


Overall stats

plate RawReads demuxcounts PCT.DMX hcReads lcReads aberrantReads PCT.Ab hcFewReads lcFewReads hcFewOverlapped lcFewOverlapped hcReported lcReported
1 TRIMMER0002_P2 571113 304026 53.23395 75408 228618 80088 35.03136 2 2 2 2 87 88
110 TRIMMER0003_P3 1684305 747844 44.40075 197906 549938 221821 40.33564 9 9 9 9 87 87
112 TRIMMER0004.2_P4 1682719 669942 39.81306 202882 467060 162326 34.75485 7 6 8 6 82 86
114 TRIMMER0005_P5 1908371 737908 38.66690 248280 489628 179969 36.75627 10 7 10 8 77 80
116 TRIMMER0006_P6 1443039 585913 40.60271 187717 398196 114406 28.73108 28 24 28 26 62 62
118 TRIMMER0007_P7 1168633 410921 35.16254 152961 257960 95433 36.99527 8 6 10 6 80 84
120 TRIMMER0010_P10R 661554 154918 23.41729 788 154130 61403 39.83845 68 60 76 60 2 32
127 TRIMMER0013_P28 1637909 586930 35.83410 167911 419019 172283 41.11580 0 0 0 0 70 90
129 TRIMMER0014_P29 1163087 257317 22.12362 88190 169127 51526 30.46586 3 1 4 1 78 89
130 TRIMMER0015_P30 860187 187889 21.84281 62289 125600 39238 31.24045 3 2 5 2 76 82
140 TRIMMER0016_P31 1492272 373133 25.00436 123295 249838 88118 35.27005 0 0 0 0 78 85
150 TRIMMER0017_P45 1637424 351926 21.49266 93414 258512 69892 27.03627 1 1 1 2 79 89
160 TRIMMER0018_P8 1359951 542317 39.87769 172102 370215 141228 38.14756 2 0 2 1 80 85
170 TRIMMER0019_P9 1251720 540508 43.18122 149384 391124 152259 38.92858 5 4 8 4 68 87
180 TRIMMER0020_P10 1382743 508309 36.76092 92486 415823 151553 36.44652 10 2 14 3 48 86
190 TRIMMER0021_P11 1387628 559219 40.30035 129104 430115 160797 37.38465 9 5 10 5 64 83
1100 TRIMMER0022_P12 1400058 600986 42.92579 200949 400037 102918 25.72712 3 2 3 3 86 92
1101 TRIMMER0023_P13 1522181 653014 42.89989 146839 506175 229933 45.42559 3 2 4 2 68 89
1102 TRIMMER0024_P14 1928710 837487 43.42213 222478 615009 223753 36.38207 9 4 12 5 52 70
1103 TRIMMER0026_P16 1378979 557860 40.45457 143772 414088 127797 30.86228 6 2 6 4 81 88
1104 TRIMMER0027_P33 870290 446766 51.33530 131748 315018 132525 42.06902 8 3 16 3 63 80
1105 TRIMMER0028_P34 766313 401893 52.44502 163282 238611 78587 32.93520 5 1 13 2 77 89
1106 TRIMMER0029_P35 865332 425476 49.16911 154320 271156 101072 37.27448 2 1 8 1 78 81
1107 TRIMMER0030_P36 1198844 591499 49.33911 209841 381658 122177 32.01217 5 1 7 1 79 89
1108 TRIMMER0031_P15 846682 379940 44.87399 131532 248408 80270 32.31377 4 2 9 2 62 76
1109 TRIMMER0033_P17 882659 432611 49.01225 165954 266657 115077 43.15544 5 2 6 2 75 83
1130 TRIMMER0034_P18 1217682 594387 48.81299 253379 341008 90832 26.63633 1 2 2 2 78 88
1132 TRIMMER0035_P19 1087896 534267 49.11012 222215 312052 89658 28.73175 3 1 4 2 84 88
1134 TRIMMER0036_P20 857227 439583 51.27965 182702 256881 91492 35.61649 7 6 8 6 78 85
1136 TRIMMER0037_P21 906420 479596 52.91101 157886 321710 106919 33.23459 5 3 12 3 68 85
1138 TRIMMER0038_P22 961787 482320 50.14832 148460 333860 131848 39.49200 3 3 5 3 83 92
1140 TRIMMER0039_P23 1012149 505116 49.90530 190468 314648 129703 41.22162 3 3 3 3 86 93
1142 TRIMMER0040_P24 713687 344704 48.29904 155448 189256 72400 38.25506 3 3 5 3 88 89
1144 TRIMMER0041_P25 878452 410411 46.71980 163635 246776 80004 32.41968 3 2 3 2 90 90
1146 TRIMMER0042_P26 817238 385514 47.17279 149642 235872 61152 25.92593 5 1 6 3 79 83
1148 TRIMMER0043_P27 941046 425255 45.18961 158740 266515 99160 37.20616 6 2 7 2 77 80
1150 TRIMMER0044_P37 1195904 783237 65.49330 262771 520466 210772 40.49679 3 2 5 2 76 93
1152 TRIMMER0045_P38 1109354 703337 63.40059 264957 438380 180199 41.10566 6 5 6 5 77 91
1154 TRIMMER0046_P39 966125 626457 64.84223 195548 430909 210318 48.80798 5 5 6 5 70 91
1156 TRIMMER0047_P40 1182635 739123 62.49798 183035 556088 236402 42.51162 6 2 12 2 63 89
1158 TRIMMER0048_P41 1243060 825608 66.41739 304705 520903 221330 42.48968 7 5 9 5 77 90
1160 TRIMMER0049_P42 1042908 705715 67.66800 245921 459794 205840 44.76787 4 3 9 4 77 88
1162 TRIMMER0050_P43 734473 495020 67.39798 177546 317474 121678 38.32692 4 3 4 3 90 92
1164 TRIMMER0051_P44 922254 618484 67.06222 219444 399040 154134 38.62620 3 2 7 2 87 93
1166 TRIMMER0052_P46 834525 559073 66.99296 159049 400024 148349 37.08502 6 4 6 4 89 92
1168 TRIMMER0053_P47 966609 648902 67.13180 208894 440008 157572 35.81117 6 3 7 3 89 90
1170 TRIMMER0054_P48 1051379 707912 67.33176 268424 439488 117992 26.84760 5 5 5 5 90 91
1172 TRIMMER0055_P49 934382 635271 67.98836 246892 388379 130664 33.64343 5 4 5 4 88 92
1174 TRIMMER0056_P50 818736 550103 67.18930 171867 378236 152996 40.44988 4 3 7 3 83 91
1176 TRIMMER0057_P51 979265 643470 65.70949 257470 386000 116605 30.20855 4 3 4 3 92 92
1178 TRIMMER0058_P52 1048688 680023 64.84512 216639 463384 167697 36.18964 2 1 2 1 92 93
1180 TRIMMER0059_P54 864904 550011 63.59214 192296 357715 146336 40.90854 2 1 2 1 92 95
1182 TRIMMER0060_P55 871030 585848 67.25922 214410 371438 147377 39.67742 3 3 4 3 91 93
1184 TRIMMER0061_P56 1000302 673345 67.31417 219625 453720 167602 36.93952 3 3 3 3 91 93
1186 TRIMMER0062_P57 1404759 926346 65.94341 383917 542429 193398 35.65407 4 3 4 4 89 92
1188 TRIMMER0063_P58 848043 517933 61.07391 206040 311893 93647 30.02536 5 2 6 3 90 91
1190 TRIMMER0064_P59 1279777 831088 64.94006 267019 564069 216245 38.33662 5 2 5 2 89 93
1192 TRIMMER0065_P60 1112888 723069 64.97231 221748 501321 219864 43.85693 3 3 4 3 83 93
1194 TRIMMER0066_P65 1130112 722677 63.94738 300747 421930 159780 37.86884 6 4 8 4 81 85
1196 TRIMMER0067_P66 761515 491951 64.60162 213764 278187 106973 38.45363 6 6 6 6 90 90
1198 TRIMMER0068_P1R 367088 124332 33.86981 41672 82660 32744 39.61287 9 5 10 5 85 91
1200 TRIMMER0069_PDP1 2337556 1507646 64.49668 209881 1297765 912057 70.27906 18 9 18 9 47 55
1201 TRIMMER0070_PDP2 2498629 1664750 66.62654 328191 1336559 878067 65.69609 6 1 8 2 53 70
1202 TRIMMER0073_PS1 2748584 1806289 65.71707 676631 1129658 495904 43.89860 7 6 8 6 81 88
1203 TRIMMER0074_PS2 3869822 2505308 64.73962 791827 1713481 694712 40.54390 9 7 9 8 82 86
1204 TRIMMER0075_PS3 3621378 2230240 61.58540 891079 1339161 495193 36.97785 3 1 3 1 89 92
1205 TRIMMER0077_P32R 2021192 1265368 62.60504 390561 874807 271337 31.01678 4 0 4 0 78 89
1206 TRIMMER0078_P67 1770696 1184371 66.88731 446632 737739 262418 35.57057 4 3 4 3 91 91
1207 TRIMMER0079_P68 1896521 1262527 66.57068 504750 757777 288438 38.06370 5 4 5 4 86 90
1208 TRIMMER0080_P69 1973427 1258260 63.76015 483539 774721 357605 46.15920 6 3 7 5 84 87
1209 TRIMMER0076_P6R 1372694 881211 64.19573 323472 557739 154176 27.64304 4 3 4 3 81 84
1273 TRIMMER0081_P70 2005190 1310164 65.33865 497153 813011 301520 37.08683 2 1 2 1 88 91
1275 TRIMMER0082_P71 1534823 998923 65.08392 414981 583942 242670 41.55721 8 3 8 3 80 85
1277 TRIMMER0083_P72 1549641 939475 60.62533 413077 526398 188531 35.81530 4 3 6 3 83 88
1279 TRIMMER0084_P73 593252 370094 62.38394 95406 274688 128749 46.87100 5 3 5 3 83 88
1281 TRIMMER0085_P74 489362 297965 60.88846 103832 194133 66371 34.18842 10 11 10 11 83 84
1283 TRIMMER0086_P75 386039 232809 60.30712 79573 153236 66373 43.31423 6 6 9 6 87 87


Samples that didn't produce an ASV in one or both chains:


Variance in reads by SMARTindex (sample) across plates

Values on the Y-axis are per-primer pair and calculated like:

100 * (number of reads with correct primer sequence)/(total number of reads for the plate).

The better the pooling and more consistently samples performed within plate, the less variance in percent reads there should be.

Optimal y-value should be ~ 0.5 because there are 96*2 barcode pairs (100/192).

The values for LC include the aberrant reads.

Percentage of reads by Primer+Index

Note that the denominator is total reads per plate.

Variance in total reads by Primer+Index

Variance in percent reads with primer by Plate

Percent of reads with Primer+Index that could be overlapped

Percent overlap should be ~100 because insert lengths are short enough that all on-target amplicons should overlap unless read quality was low. There should also be very low plate-to-plate or sample-to-sample variance in overlap unless read quality was low, there were a large number of primer dimers or something else went wrong.

ASVs supported by 10 or more reads

Number of HC ASVs with support from 10 or more reads:

In plates containing monoclonal samples, each sample should contain one heavy chain and two light chain sequences. Plates that contain non-monoclonal (parent) samples will have multiple sequences. The values reported here represent pre-filtered ASV counts for ASVs with more than 10 supporting reads.

Number of HC ASVs reported:

This plot shows the distribution of number of HC ASVs reported after filtering per plate.

Number of LC ASVs with support from 10 or more reads:

In plates containing monoclonal samples, each sample should contain one heavy chain and two light chain sequences. Plates that contain non-monoclonal (parent) samples will have multiple sequences. The values reported here represent pre-filtered ASV counts for ASVs with more than 10 supporting reads.

Number of LC ASVs reported:

This plot shows the distribution of number of LC ASVs reported after filtering per plate.

Other plots..

Light chain

Count of overlapped reads, samples with < 10 reads are Red, samples with 10-50 reads a yellow, samples with > 50 reads are green.

Heavy Chain

Count of overlapped reads, samples with < 10 reads are Red, samples with 10-50 reads a yellow, samples with > 50 reads are green.

Duplicate ASVs

See TSV file for duplicate results.